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pegfpc1 expression vector  (Addgene inc)


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    Structured Review

    Addgene inc pegfpc1 expression vector

    Pegfpc1 Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/expression+vector+pegfpc1/pEGFPC1-6XHis-FLKSRP+(Plasmid+%2323001)/pmc11149935-88-12-10
    Average 92 stars, based on 4 article reviews
    pegfpc1 expression vector - by Bioz Stars, 2026-10
    92/100 stars

    Images

    1) Product Images from "SAFB regulates hippocampal stem cell fate by targeting Drosha to destabilize Nfib mRNA"

    Article Title: SAFB regulates hippocampal stem cell fate by targeting Drosha to destabilize Nfib mRNA

    Journal: eLife

    doi: 10.7554/eLife.74940


    Figure Legend Snippet:

    Techniques Used: Protease Inhibitor, Recombinant, Clone Assay, Bicinchoninic Acid Protein Assay, Mutagenesis, Transfection, Sequencing, esiRNA, Blocking Assay, Plasmid Preparation, Expressing, Software

    Related Articles

    Recombinant:

    Article Title: SAFB regulates hippocampal stem cell fate by targeting Drosha to destabilize Nfib mRNA
    Article Snippet: Recombinant DNA reagent , pEGFP-DHX9 (plasmid) , , https://doi.org/10.18632/oncotarget.5033 , pEGFP expression vector. .. Recombinant DNA reagent , pEGFPC1-6XHis- FLKSRP (plasmid) , , RRID: Addgene_23001 , pEGFPC1 expression vector. .. Recombinant DNA reagent , pENTR-D-Topo- hTRIM9 (plasmid) , , RRID: Addgene_51032 , pENTR expression vector.

    Plasmid Preparation:

    Article Title: SAFB regulates hippocampal stem cell fate by targeting Drosha to destabilize Nfib mRNA
    Article Snippet: Recombinant DNA reagent , pEGFP-DHX9 (plasmid) , , https://doi.org/10.18632/oncotarget.5033 , pEGFP expression vector. .. Recombinant DNA reagent , pEGFPC1-6XHis- FLKSRP (plasmid) , , RRID: Addgene_23001 , pEGFPC1 expression vector. .. Recombinant DNA reagent , pENTR-D-Topo- hTRIM9 (plasmid) , , RRID: Addgene_51032 , pENTR expression vector.

    Expressing:

    Article Title: SAFB regulates hippocampal stem cell fate by targeting Drosha to destabilize Nfib mRNA
    Article Snippet: Recombinant DNA reagent , pEGFP-DHX9 (plasmid) , , https://doi.org/10.18632/oncotarget.5033 , pEGFP expression vector. .. Recombinant DNA reagent , pEGFPC1-6XHis- FLKSRP (plasmid) , , RRID: Addgene_23001 , pEGFPC1 expression vector. .. Recombinant DNA reagent , pENTR-D-Topo- hTRIM9 (plasmid) , , RRID: Addgene_51032 , pENTR expression vector.

    other:

    Article Title: Non-canonical Wnt signaling regulates junctional mechanocoupling during angiogenic collective cell migration
    Article Snippet: DOI: https://doi.org/10.7554/eLife.45853 21 of 35 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Sequencebased reagent ON-TARGET human siRNAs Dharmacon See Table 1 Peptide, recombinant protein Recombinant human Wnt5a protein R and D Systems Cat#: 645-WN Commercial assay or kit BCA protein assay kit VWR Cat#: 786–0000 Commercial assay or kit Cdc42 Pull-down Activation Assay Biochem Kit Cytoskeleton Cat#: BK034 Commercial assay or kit Duolink In Situ Red Mouse/Rabbit Starter Kit Sigma-Aldrich Cat#: DUO92101 Commercial assay or kit ECL Western Blotting Detection Reagent GE Healthcare Cat#: RPN2232 Commercial assay or kit GeneJet RNA Purification Kit Thermo Fisher Scientific Cat#: K0731 Commercial assay or kit RNeasy Mini Kit Qiagen Cat#: 74104 Commercial assay or kit Superscript IV First-Strand Synthesis System Invitrogen Cat#: 18091050 Chemical compound, drug Dabco (1,4-Diazabicyclo [2.2.2]octane) Sigma-Aldrich Cat#: D27802 Chemical compound, drug DharmaFECT one reagent Dharmacon Cat#: T-2001–02 Chemical compound, drug DNase I NZYTech Cat#: MB19901 Chemical compound, drug DSP (dithiobis (succinimidyl propionate)) Alfagene Cat#: 22585 Chemical compound, drug Fibronectin Sigma-Aldrich Cat#: F1141 Chemical compound, drug Full Range Rainbow Recombinant protein Molecular weight marker GE Healthcare Cat#: RPN800E Chemical compound, drug Gelatin Sigma-Aldrich Cat#: G1393 Chemical compound, drug ML-141 Sigma-Aldrich Cat#: SML0407 10 mM Chemical compound, drug Mowiol Sigma-Aldrich Cat#: 81381 Chemical compound, drug NSC 23766 Tocris Cat#: 2161 100 mM Chemical compound, drug Phosphatase and proteinase inhibitors cocktail Thermo Fisher Scientific Cat#: 1861281 Chemical compound, drug Pierce G-protein agarose beads Thermo Fisher Scientific Cat#: 22851 Chemical compound, drug Ponceau Red NZYTech Cat#: MB19201 Continued on next page Carvalho et al. eLife 2019;8:e45853.



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    Figure 2: Co-immunoprecipitation experiments in HEK293 cells transfected with (A) pEF-BOS-SNAP25-FLAG alone or together with <t>pEGFPC1-TrkB.</t> (B) pEF-BOS-VAMP2-FLAG alone or together with pEGFPC1-TrkB. (C) pEF-BOS-SNAP25-FLAG, pEF-BOS- VAMP2-FLAG and TrkA-HA. (D) pEF-BOS-SNAP25-FLAG, pEF-BOS-VAMP2-FLAG and TrkC-myc. No co-immunoprecipitation was observed between proteins analyzed with anti α-GFP, anti-myc, anti-FLAG, or anti-HA antibodies. Two transfections in HEK cells per condition were run in parallel for each experiment and three experiments were done. Arrows indicate specific bands.
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    Image Search Results


    Figure 2: Co-immunoprecipitation experiments in HEK293 cells transfected with (A) pEF-BOS-SNAP25-FLAG alone or together with pEGFPC1-TrkB. (B) pEF-BOS-VAMP2-FLAG alone or together with pEGFPC1-TrkB. (C) pEF-BOS-SNAP25-FLAG, pEF-BOS- VAMP2-FLAG and TrkA-HA. (D) pEF-BOS-SNAP25-FLAG, pEF-BOS-VAMP2-FLAG and TrkC-myc. No co-immunoprecipitation was observed between proteins analyzed with anti α-GFP, anti-myc, anti-FLAG, or anti-HA antibodies. Two transfections in HEK cells per condition were run in parallel for each experiment and three experiments were done. Arrows indicate specific bands.

    Journal: Oncotarget

    Article Title: Syntaxin-1/TI-VAMP SNAREs interact with Trk receptors and are required for neurotrophin-dependent outgrowth.

    doi: 10.18632/oncotarget.26307

    Figure Lengend Snippet: Figure 2: Co-immunoprecipitation experiments in HEK293 cells transfected with (A) pEF-BOS-SNAP25-FLAG alone or together with pEGFPC1-TrkB. (B) pEF-BOS-VAMP2-FLAG alone or together with pEGFPC1-TrkB. (C) pEF-BOS-SNAP25-FLAG, pEF-BOS- VAMP2-FLAG and TrkA-HA. (D) pEF-BOS-SNAP25-FLAG, pEF-BOS-VAMP2-FLAG and TrkC-myc. No co-immunoprecipitation was observed between proteins analyzed with anti α-GFP, anti-myc, anti-FLAG, or anti-HA antibodies. Two transfections in HEK cells per condition were run in parallel for each experiment and three experiments were done. Arrows indicate specific bands.

    Article Snippet: Full-length Sytx1A or truncated DNAs (Sytx1 ACYT, Sytx1AHabc, Sytx1AH3TM, Sytx1AH3, and Sytx1ATM) were cloned into EcoRI sites in pEGFPC1 fusion mammalian protein expression vector (Clontech), as described [18].

    Techniques: Immunoprecipitation, Transfection

    Journal: eLife

    Article Title: SAFB regulates hippocampal stem cell fate by targeting Drosha to destabilize Nfib mRNA

    doi: 10.7554/eLife.74940

    Figure Lengend Snippet:

    Article Snippet: Recombinant DNA reagent , pEGFPC1-6XHis- FLKSRP (plasmid) , , RRID: Addgene_23001 , pEGFPC1 expression vector.

    Techniques: Protease Inhibitor, Recombinant, Clone Assay, Bicinchoninic Acid Protein Assay, Mutagenesis, Transfection, Sequencing, esiRNA, Blocking Assay, Plasmid Preparation, Expressing, Software