pegfpc1 expression vector (Addgene inc)
Structured Review

Pegfpc1 Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/expression+vector+pegfpc1/pEGFPC1-6XHis-FLKSRP+(Plasmid+%2323001)/pmc11149935-88-12-10
Average 92 stars, based on 4 article reviews
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1) Product Images from "SAFB regulates hippocampal stem cell fate by targeting Drosha to destabilize Nfib mRNA"
Article Title: SAFB regulates hippocampal stem cell fate by targeting Drosha to destabilize Nfib mRNA
Journal: eLife
doi: 10.7554/eLife.74940
Figure Legend Snippet:
Techniques Used: Protease Inhibitor, Recombinant, Clone Assay, Bicinchoninic Acid Protein Assay, Mutagenesis, Transfection, Sequencing, esiRNA, Blocking Assay, Plasmid Preparation, Expressing, Software
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Recombinant:Article Title: SAFB regulates hippocampal stem cell fate by targeting Drosha to destabilize Nfib mRNA Article Snippet: Recombinant DNA reagent , pEGFP-DHX9 (plasmid) , , https://doi.org/10.18632/oncotarget.5033 , pEGFP expression vector. .. Recombinant DNA reagent , pEGFPC1-6XHis- FLKSRP (plasmid) , , RRID: Addgene_23001 , Plasmid Preparation:Article Title: SAFB regulates hippocampal stem cell fate by targeting Drosha to destabilize Nfib mRNA Article Snippet: Recombinant DNA reagent , pEGFP-DHX9 (plasmid) , , https://doi.org/10.18632/oncotarget.5033 , pEGFP expression vector. .. Recombinant DNA reagent , pEGFPC1-6XHis- FLKSRP (plasmid) , , RRID: Addgene_23001 , Expressing:Article Title: SAFB regulates hippocampal stem cell fate by targeting Drosha to destabilize Nfib mRNA Article Snippet: Recombinant DNA reagent , pEGFP-DHX9 (plasmid) , , https://doi.org/10.18632/oncotarget.5033 , pEGFP expression vector. .. Recombinant DNA reagent , pEGFPC1-6XHis- FLKSRP (plasmid) , , RRID: Addgene_23001 , other:Article Title: Non-canonical Wnt signaling regulates junctional mechanocoupling during angiogenic collective cell migration Article Snippet: DOI: https://doi.org/10.7554/eLife.45853 21 of 35 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Sequencebased reagent ON-TARGET human siRNAs Dharmacon See Table 1 Peptide, recombinant protein Recombinant human Wnt5a protein R and D Systems Cat#: 645-WN Commercial assay or kit BCA protein assay kit VWR Cat#: 786–0000 Commercial assay or kit Cdc42 Pull-down Activation Assay Biochem Kit Cytoskeleton Cat#: BK034 Commercial assay or kit Duolink In Situ Red Mouse/Rabbit Starter Kit Sigma-Aldrich Cat#: DUO92101 Commercial assay or kit ECL Western Blotting Detection Reagent GE Healthcare Cat#: RPN2232 Commercial assay or kit GeneJet RNA Purification Kit Thermo Fisher Scientific Cat#: K0731 Commercial assay or kit RNeasy Mini Kit Qiagen Cat#: 74104 Commercial assay or kit Superscript IV First-Strand Synthesis System Invitrogen Cat#: 18091050 Chemical compound, drug Dabco (1,4-Diazabicyclo [2.2.2]octane) Sigma-Aldrich Cat#: D27802 Chemical compound, drug DharmaFECT one reagent Dharmacon Cat#: T-2001–02 Chemical compound, drug DNase I NZYTech Cat#: MB19901 Chemical compound, drug DSP (dithiobis (succinimidyl propionate)) Alfagene Cat#: 22585 Chemical compound, drug Fibronectin Sigma-Aldrich Cat#: F1141 Chemical compound, drug Full Range Rainbow Recombinant protein Molecular weight marker GE Healthcare Cat#: RPN800E Chemical compound, drug Gelatin Sigma-Aldrich Cat#: G1393 Chemical compound, drug ML-141 Sigma-Aldrich Cat#: SML0407 10 mM Chemical compound, drug Mowiol Sigma-Aldrich Cat#: 81381 Chemical compound, drug NSC 23766 Tocris Cat#: 2161 100 mM Chemical compound, drug Phosphatase and proteinase inhibitors cocktail Thermo Fisher Scientific Cat#: 1861281 Chemical compound, drug Pierce G-protein agarose beads Thermo Fisher Scientific Cat#: 22851 Chemical compound, drug Ponceau Red NZYTech Cat#: MB19201 Continued on next page Carvalho et al. eLife 2019;8:e45853. |
